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To detect naturally occurring bioactive compounds in unused marine resources such as marine by-products and algae, the screening test for the antimicrobial compounds containing in Asterina pectinifera, Halocynthia rotetzi skin, Nototodarus sloani ink. Anthocidaris rrassispina skin, and four algae(Sargassum horneri, Agarum cribrosum, Odonthalia corymbifera and Desmarestia ligulata) was carried out. The minimum inhibitory concentrations(MIC, μg/disk) of ether and methanol fractions extracted from Sargassum horneri against Bacillus subtilis were 80 and 70 μg/disk, respectively. The MICs of the ether, acetone and methanol fractions extracted from Sargassum horneri against Staphylococcus aureus were 160, 180 and 70 μg/disk, respectively. In addition, the MICs of ether and acetone fractions extracted from Odonthalia corymbifera against Staphylococcus aureus were 50 and 170 μg/disk, respectively. These nonpolar fractions showed the strongest antimicrobial effect among several fractions extracted from tested marine resources.
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